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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,785 results for: emb

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Showing: 7021 - 7040 of 11785 results

  1. pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1

    Plasmid
    #250911
    Purpose
    Dual color expression of mCherry tagged human Sec 61B and EGFP tagged zebrafish LaminB1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    mCherry-Sec61B-P2A-EGFP-LMNB1 (SEC61B, lmnb1 Human, Zebrafish)
    Tags
    EGFP on LMNB1 and mCherry on Sec61b
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250911-plasmid-map-sequence-id-501821

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  3. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Publication
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Photinus pyralis, Synthetic)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  9. pelB-MBP-P13-ss

    Plasmid
    #137065
    Purpose
    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P13: lacks the P13 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137065-plasmid-map-sequence-id-291955

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  17. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Publication
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7021 - 7040 of 11785 results