We narrowed to 9,672 results for: Pol

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCBS-5753

Plasmid

#249325

Purpose

To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC dDarT2(DLAA)-nScCas9(D10A)

Expression

Yeast

Mutation

DarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTB106-Hyg

Plasmid

#236779

Purpose

Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Insert

hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP

Use

CRISPR

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTB107-Phleo

Plasmid

#236782

Purpose

Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Insert

hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP

Use

CRISPR

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-Flag-SREBP-1c

Plasmid

#237348

Purpose

Adenoviral-SREBP-1c cleavage-activation reporter system

Depositor

Gerald Höfler

Article

Pena de la Sancha et al Commun Biol. 2025 Apr 9;8(1):588. doi: 1

Insert

SREBF1 (SREBF1 Human)

Use

Adenoviral and Mouse Targeting

Tags

Flag tag

Promoter

CMV

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-1α-Venus-Nup107-FRT

Plasmid

#247344

Purpose

Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

Nup107 (NUP107) (NUP107 Human)

Tags

Venus

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1 T62 H67 M68

Plasmid

#246506

Purpose

Recombinant protein expression of PCMTD1 T62 H67 M68

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_4

Plasmid

#242899

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_1

Plasmid

#242897

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_3

Plasmid

#242898

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TCTN3 minigene (pAVA3939)

Plasmid

#239345

Purpose

Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)

Tags

3xFLAG, 6xMyc, and CopGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Rab11 single guide RNA

Plasmid

#229683

Purpose

guide RNA for Rab11 tagging at the N-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

Rab11 guide (RAB11A Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS_RRM-RGG(359-513)

Plasmid

#188046

Purpose

Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 514-656

Promoter

T7

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

Plasmid

#217802

Purpose

Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

Plasmid

#217806

Purpose

Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

Plasmid

#217790

Purpose

Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

Plasmid

#217791

Purpose

Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

Plasmid

#217792

Purpose

Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

Plasmid

#217793

Purpose

Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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