161,697 results

1. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV5)

   Viral Prep

   #26969-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Promoter

   CaMKII

   Tags

   EYFP

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

   Viral Prep

   #105551-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   GFP

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

4. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

   Plasmid

   #204146

   Purpose

   Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Inserts

   SARS-CoV-2 BA.1 variant spike
   zsGreen-T2A-PuR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   CMV and TRE3G

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

   Viral Prep

   #198513-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   Syn

   Tags

   EGFP

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pExp-Trx

    Plasmid

    #112577

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-TrxA and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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