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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,030 results for: Pol

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Showing: 7081 - 7100 of 10030 results

  1. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid
    #193719
    Purpose
    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
    Tags
    2xHA-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193719-plasmid-map-sequence-id-383790

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  2. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109425-plasmid-map-sequence-id-212947

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  8. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  17. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid
    #208976
    Purpose
    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    nSpCas9(H840)-BPNLS-P2A-GFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    208976-plasmid-map-sequence-id-415837

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Showing: 7081 - 7100 of 10030 results