We narrowed to 12,024 results for: pcdna

1. pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

   Plasmid

   #65847

   Purpose

   Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R5D-K6D

   Promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

   Plasmid

   #59827

   Purpose

   Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc Separase delta 1278-1291

   Plasmid

   #59830

   Purpose

   Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 1278-1291

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

   Plasmid

   #52129

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-470 and changed C478S, C667…

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

   Plasmid

   #52130

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-470 and changed C478S, C643…

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

   Plasmid

   #52131

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-470 and changed C478S, C631…

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

   Plasmid

   #50866

   Purpose

   Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C I730C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

   Plasmid

   #50870

   Purpose

   Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   N680C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

   Plasmid

   #50867

   Purpose

   Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)

    Plasmid

    #50864

    Purpose

    Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)

    Plasmid

    #50869

    Purpose

    Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S679C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)

    Plasmid

    #50863

    Purpose

    Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

    Plasmid

    #50868

    Purpose

    Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I677C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid

    #49080

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C791S, C820S, C1052S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid

    #49070

    Purpose

    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    ECL4 deletion of amino acids 562-582 in hNKCC1 in…

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)

    Plasmid

    #49073

    Purpose

    Expresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C723S,C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid

    #49062

    Purpose

    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    N-terminal deletion of amino acids 13-222 in hNKC…

    Promoter

    CMV

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

    Plasmid

    #111460

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346A, C643A

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

    Plasmid

    #111461

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346D, C643A

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113612

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    S1P1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge