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Ad-HisFlag-USP19ER

Plasmid

#155244

Purpose

for generating adenoviruses expressing the ER isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Mutation

H118R- please see dep. comments

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPN018

Plasmid

#91664

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN282

Plasmid

#91665

Purpose

Express sgRNA targeting human SDCCAG8

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SDCCAG8 (SDCCAG8 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN283

Plasmid

#91666

Purpose

Express sgRNA targeting human SDCCAG8

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SDCCAG8 (SDCCAG8 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN150

Plasmid

#91679

Purpose

Express sgRNA targeting human TSNARE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TSNARE1 (TSNARE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN151

Plasmid

#91680

Purpose

Express sgRNA targeting human TSNARE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TSNARE1 (TSNARE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN272

Plasmid

#91643

Purpose

Express sgRNA targeting human MMP16

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MMP16 (MMP16 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN273

Plasmid

#91644

Purpose

Express sgRNA targeting human MMP16

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MMP16 (MMP16 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN278

Plasmid

#91653

Purpose

Express sgRNA targeting human PTN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTN (PTN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN279

Plasmid

#91654

Purpose

Express sgRNA targeting human PTN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTN (PTN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN017

Plasmid

#91663

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN176

Plasmid

#91627

Purpose

Express sgRNA targeting human IDO2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

IDO2 (IDO2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN014

Plasmid

#91588

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2mut3

Plasmid

#53742

Purpose

promoter region of PIM2 lacking the 2 forkhead binding sites

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Mutation

deletion of 2 forkhead binding sites (bp# 395-497…

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pX41

Plasmid

#46851

Purpose

TOP Hupki-wt plasmid without selection marker

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB and Hupki p53 wild type (TP53 Human, Mouse)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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