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GB3408

Plasmid

#193105

Purpose

Minimal promoter of slCHS1 gene (Solyc09g091510) containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlCHS

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3414

Plasmid

#193111

Purpose

Minimal promoter of AnGPDA gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB007 sequence).

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minAnGPDA

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3271

Plasmid

#193121

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.7

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2566

Plasmid

#193104

Purpose

Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlDFR

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3413

Plasmid

#193110

Purpose

Minimal promoter of PcPAF gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB029 sequence).

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minPcPAF

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2884

Plasmid

#193122

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.2

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3274

Plasmid

#193136

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA3 (GB1724) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G3aG2b.1

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2889

Plasmid

#193127

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.5

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3272

Plasmid

#193134

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1b.1

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2891

Plasmid

#193128

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.1

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB2885

Plasmid

#193123

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.1

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB2879

Plasmid

#193116

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.5

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3278

Plasmid

#193132

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.5

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3273

Plasmid

#193135

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1e.1

Use

Synthetic Biology

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAGE1.0

Plasmid

#165983

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

Depositor

Bogumil Karas

Publication

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
spectinomycin/streptomycin resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-145

Plasmid

#185934

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Publication

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY227

Plasmid

#184148

Purpose

short crRNA generator with spacer LEA2 with WT repeat region

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-crRNA-LEA2-WT

Use

Synthetic Biology

Promoter

Plux2

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-135

Plasmid

#185926

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Publication

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::HA-RfA

Plasmid

#186404

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsSMG7opt_732-1091_V

Plasmid

#147769

Purpose

Bacterial Expression of HsSMG7opt_732-1091

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSMG7opt_732-1091 (SMG7 Human)

Expression

Bacterial

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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