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Plasmid#60577Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_5
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtS1R1R2B2
Plasmid#160882PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA
Plasmid#133339Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-AtC60αβC20NtL
Plasmid#160876PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltalinker
Plasmid#157870Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the linkerDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltalinerPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK446 (BSD-P2A-BromoTag-mClover)
Plasmid#214376PurposeBromoTag-mClover for N-terminal taggingDepositorInsertBSD-P2A-BromoTag-mClover
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK447 (BSD-P2A-BromoTag-mCherry)
Plasmid#214377PurposeBromoTag-mCherry for N-terminal taggingDepositorInsertBSD-P2A-BromoTag-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaCTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSExpressionMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVCERC-5 Pvan-dcas9 (Sth3)
Plasmid#133319Purposeexpresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromotervanillateAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK282 (mCherry2-Bsr)
Plasmid#72798PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMGA-plux-sfGFP
Plasmid#139936PurposeAHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLuxR-Plux-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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NTM (FLM-PC1Δ137)
Plasmid#41572DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationsequence of PKD1 FLM from 4078 - 4273PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-NtC60αC20NtLC60β
Plasmid#160877PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ30
Plasmid#41568DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ30; sequence of PKD1 from 4078 - 4273PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ45
Plasmid#41569DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ45; sequence of PKD1 from 4078 - 4258PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ67
Plasmid#41570DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ67; sequence of PKD1 from 4078 - 4236PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ103
Plasmid#41571DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ103; sequence of PKD1 from 4078 - 4200PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralTagsExpressionMutationmCherry has M9S, M16L to reduce background mCherr…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only