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pNF02-mLemon

Plasmid

#219399

Purpose

Constitutive expression of mLemon in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLemon

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-4xnrUAS (L4-R1)

Plasmid

#61372

Purpose

5' Gateway Entry Clone Containing 4xnrUAS

Depositor

Marnie Halpern

Article

Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

Insert

4xnrUAS

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-CLV3

Plasmid

#228697

Purpose

Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 SENSOR

Use

Synthetic Biology and Unspecified

Tags

mClover3

Expression

Mammalian

Promoter

hpgk

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MC2

Plasmid

#228698

Purpose

Control cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 CONTROL

Use

Synthetic Biology and Unspecified

Tags

mCherry

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Promoter

dHsp70

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Tre3G-47xCAG-12xMS2

Plasmid

#99148

Purpose

Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

47xCAG 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK-DN1

Plasmid

#180282

Purpose

Cas9 cassette (TU1)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS - pPGK1 - Cas9 - tPGK1 - ConR1

Expression

Bacterial

Promoter

pPGK1

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK-DN2

Plasmid

#180283

Purpose

Spacer cassette (TU3)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL2 - Spacer - ConRE

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSC142

Plasmid

#122835

Purpose

SUC2 promoter with GreenGate A-B flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SUC2 promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MR3

Plasmid

#228700

Purpose

Control cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 CONTROL

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR-EASY

Plasmid

#241237

Purpose

CRISPR-Cas9 genome editing

Depositor

Tamara O'Connor

Article

Hossain et al PLoS One. 2024 Mar 14;19(3):e0299513. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PPDK

Plasmid

#64037

Purpose

Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

Depositor

Vern Schramm

Article

Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

Insert

pyruvate phosphate dikinase

Tags

none

Expression

Bacterial

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDSG358

Plasmid

#115599

Purpose

surface-displayed Ag1; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-LEU2MX6

Plasmid

#33138

Purpose

gene deletion in S. cerevisiae and S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Insert

LEU2MX6

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAd5-B5

Plasmid

#122555

Purpose

Block 5 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 17998-25062

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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