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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Kan

Plasmid

#188928

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Kan

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-URA

Plasmid

#188927

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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JME5000

Plasmid

#129661

Purpose

EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMB-TGNVHAr

Plasmid

#61179

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-GAr

Plasmid

#61173

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-MITr

Plasmid

#61199

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN41r

Plasmid

#61177

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-LEr

Plasmid

#61186

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-ERr

Plasmid

#61171

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET-15b_T. maritima His-tRNase Z

Plasmid

#242436

Purpose

Expresses T. maritima tRNase Z in E. coli.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

tRNase Z

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PmxaF

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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