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Plasmid#193370PurposenTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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E2_pENTR_R4-R3_HSPter-RPS5A-NLS
Plasmid#193371PurposenTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_CYO1 exon1_1397NC
Plasmid#193376PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_CYO1 exon2_1397NC
Plasmid#193377PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_PKT3 exon1_1397NC
Plasmid#193378PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtPKT3.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_PKT3 exon12_1397NC
Plasmid#193379PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtPKT3.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_MSH1 exon5_1397NC
Plasmid#193380PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_MSH1 exon21_1397NC
Plasmid#193381PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXMA L1A
Plasmid#153347PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-paGFP-bPAC
Plasmid#204670PurposeMammalian expression of bPAC tagged with paGFP for cAMP productionDepositorArticleInsertpaGFP-bPAC
UseLentiviralTags10xHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)
Plasmid#203542PurposeMake FIP200 NTD:ATG13:ULK1 complexDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199487PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199488PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199489PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199497PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Landing_Pad_8xCTS-ECFP-pA
Plasmid#200242PurposeBackbone for cloning 8xCTSs (8xCRISPR-target sites)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-TM2Emp-6T_iBlue-ModFlanks
Plasmid#200236PurposeBackbone for cloning longer RNA triggers that activate iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZ3 dFE
Plasmid#190144PurposeExpresses LZ3Cas9-T4pol (LZ3 dFE)DepositorInsertLZ3Cas9-T4pol (LZ3 dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi dFE
Plasmid#190142PurposeExpresses HiFiCas9-T4pol (HiFi dFE)DepositorInsertHiFi Cas9-T4pol (HiFi dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationSpCas9 R691A mutant, Codon Optimized for mammalia…PromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only