Addgene

We narrowed to 3,073 results for: golden gate

Showing: 701 - 720 of 3073 results

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pICH87644

Plasmid

#50274

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM5331

Plasmid

#50294

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM1482

Plasmid

#50295

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + mitochondrial localisation signal ScCoxIV (Saccharomyces cerevisiae)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45195

Plasmid

#50275

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH85281

Plasmid

#50272

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM5343

Plasmid

#50297

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDM042

Plasmid

#216812

Purpose

Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mScarlet3

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM043

Plasmid

#216813

Purpose

Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mScarlet3

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM052

Plasmid

#216814

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM053

Plasmid

#216815

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Use

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM070

Plasmid

#216816

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Use

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM071

Plasmid

#216817

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Use

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM069

Plasmid

#216821

Purpose

Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold2

Use

Tags

RIPGLIN and VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS003

Plasmid

#194014

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

C16 Ex4 XTR f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Xalb_I-F1_array_GG

Plasmid

#178770

Purpose

Golden Gate vector to generate X. albilineans type I-F1 single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Xalb_I-C_array_GG

Plasmid

#178767

Purpose

Golden Gate vector to generate X. albilineans type I-C single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge