We narrowed to 150,751 results for: ins

1. pGEX4T-1 Slp2-a C2AB

   Plasmid

   #40058

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a C2AB (Sytl2 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C2AB domains only

   Promoter

   Tac

   Available Since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

2. NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75234

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75235

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Mist-SH2

   Plasmid

   #46454

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Mist (Clnk Mouse)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 300-435

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-mVenus-TEV

   Plasmid

   #105774

   Purpose

   Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mVenus-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hook3(1-522)-GFP-Psc-StrepII

   Plasmid

   #111861

   Purpose

   C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   Hook3(1-552)-GFP (HOOK3 Human)
   

   Tags

   GFP, Psc tag, and Strep tag

   Expression

   Insect

   Mutation

   Optimised for expression in SF9 cells

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1920

   Plasmid

   #82562

   Purpose

   High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple253
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Available Since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Hy_pMT Laccase2 MCS-HIPK3 Exon

   Plasmid

   #69888

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (HIPK3 Human, Fly)
   

   Expression

   Insect

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS1[GalS-T,TreR-T]

   Plasmid

   #60775

   Purpose

   Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   GalS-T
   TreR-T
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. TAN DBD with GalS LB…

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS1[RbsR-T,TreR-T]

    Plasmid

    #60776

    Purpose

    Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    RbsR-L
    TreR-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. TAN DBD with RbsR LB…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1941

    Plasmid

    #82564

    Purpose

    High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple264
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1 Slp2-a C2A

    Plasmid

    #40052

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a C2A (Sytl2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A domains

    Promoter

    CMV promoter

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pEf1a-Dn29-dCas9-P2A-GFP

    Plasmid

    #247155

    Purpose

    Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

    Depositor

    Patrick Hsu

    Article

    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

    Insert

    Dn29-dCas9-P2A-GFP
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-p120RasGAP-581-1047-WT

    Plasmid

    #246487

    Purpose

    Expresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coli

    Depositor

    Titus Boggon

    Article

    Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

    Insert

    RASA1 (RASA1 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-580

    Promoter

    T7lac

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b His SUMO mNeon L66R PPM1H

    Plasmid

    #240064

    Purpose

    Expresses His SUMO mNeon (inserted in loop 1) L66R PPM1H phosphtase in bacterial cells

    Depositor

    Suzanne Pfeffer

    Article

    Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

    Insert

    His SUMO mNeon (loop 1) L66R PPM1H (PPM1H Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b His SUMO mNeon Δ37 PPM1H

    Plasmid

    #240065

    Purpose

    Expresses His SUMO mNeon (inserted in loop1) Δ37 PPM1H in bacterial cells

    Depositor

    Suzanne Pfeffer

    Article

    Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

    Insert

    His SUMO mNeon (loop 1) Δ37 PPM1H (PPM1H Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAS-sfGFP-TurboID-ER

    Plasmid

    #240232

    Purpose

    Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BIP-sfGFP-TurboID-KDEL
    

    Expression

    Insect

    Promoter

    UAS

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAS-CG6867-sfGFP

    Plasmid

    #240237

    Purpose

    Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    CG6867 (CG6867 Fly)
    

    Tags

    sfGFP

    Expression

    Insect

    Promoter

    UAS

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR_hhN-HA

    Plasmid

    #240225

    Purpose

    Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Use

    Gateway shuttle vector

    Tags

    HA-spGFP11

    Mutation

    aa 1-257

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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