We narrowed to 7,723 results for: ALP

1. pGAL4-DBD-CEBPa_WT(N)-IS15

   Plasmid

   #215636

   Purpose

   For overexpression of Gal4-DBD CEBPa WT(N) IS15

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   CEBPa(N) IS15 (CEBPA Human)
   

   Expression

   Mammalian

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAL4-DBD-CEBPa-AroPERFECT_IS10

   Plasmid

   #215606

   Purpose

   For overexpression of Gal4-DBD CEBPa AroPERFECT IS10

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   CEBPa AroPERFECT IS10 (CEBPA Human)
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAL4-DBD-CEBPa-AroLITE_A

   Plasmid

   #215602

   Purpose

   For overexpression of Gal4-DBD CEBPa AroLITE

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   CEBPa AroLITE (CEBPA Human)
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. B2M-SDMutation-EhT

   Plasmid

   #215546

   Purpose

   Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
   

   Use

   CRISPR

   Mutation

   The second base of the first intron of B2M (From …

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. B2M-SDMutation-EPG

   Plasmid

   #215545

   Purpose

   Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
   

   Use

   CRISPR

   Mutation

   The second base of the first intron of B2M (From …

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W

   Plasmid

   #211988

   Purpose

   Express gRNA against TRIM24 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting TRIM24 (TRIM24 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(TRIM24_5-4)-PGKpuroBFP-W

   Plasmid

   #211989

   Purpose

   Express gRNA against TRIM24 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting TRIM24 (TRIM24 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bn R52A, R54A pMT2

   Plasmid

   #206087

   Purpose

   Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   R52A, R54A

   Promoter

   Ad MLP/TPL/SV40

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.2 W391A pcDNA3

   Plasmid

   #206085

   Purpose

   expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1605

    Plasmid

    #143530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BAZ2A (BAZ2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0003

    Plasmid

    #142141

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF3A (HIF3A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5 Myc-tagged PSK (K57A)

    Plasmid

    #197150

    Purpose

    Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

    Insert

    TAOK2 (TAOK2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    SV40

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgCtrl_EF1a-Puro-T2A-BFP

    Plasmid

    #195500

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

    Plasmid

    #195501

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0004

    Plasmid

    #141471

    Purpose

    Lentiviral vector for overexpressing the HIF3A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF3A (HIF3A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid

    #182887

    Purpose

    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    silet mutations in aa 197 - 203 to confer resista…

    Promoter

    EF1alpha core

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid

    #182885

    Purpose

    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    stop codon introduced 2 amino acids downstream of…

    Promoter

    EF1alpha core

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

    Plasmid

    #185717

    Purpose

    AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Mutation

    Changed Ala 11 to Pro, Val 70 to Pro

    Promoter

    hSyn

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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