We narrowed to 14,429 results for: nts

1. pMX GFP cdc42 Q61L

   Plasmid

   #14568

   Depositor

   Joan Brugge

   Article

   Hall et al Immunity. 2006 Mar . 24(3):305-16.

   Insert

   cdc42 Q61L (CDC42 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L

   Promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA_DiCre_60.F2

   Plasmid

   #60208

   Purpose

   Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
   

   Use

   Tags

   FRAP and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 2021-2112 of FRAP with a T2098L mutat…

   Promoter

   CMV

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-A119S

   Plasmid

   #177948

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (A119S) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A119S

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. plc242-GFP-ITFG2

   Plasmid

   #87049

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJG367

   Plasmid

   #91185

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_H840A+gNt_R2+gNt_F2+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJG382

   Plasmid

   #91189

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gNt_R2+gNt_F2+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-Epsin 2

   Plasmid

   #22241

   Depositor

   Pietro De Camilli

   Article

   Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

   Insert

   Epsin 2 (EPN2 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-6P-2_dCrk

   Plasmid

   #131146

   Purpose

   Expresses GST::dCrk (based on crk isoform RA).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLINTR BFP v1 backbone

    Plasmid

    #179779

    Purpose

    SPLINTR backbone vector

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. DCT.VV.Orf107

    Plasmid

    #12795

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf107
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. DCT.VV.Orf37

    Plasmid

    #12725

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf37
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. DCT.VV.Orf48

    Plasmid

    #12736

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf48
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

16. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBSFI-myr-akt-T308A

    Plasmid

    #49189

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197966

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. VQR variant

    Plasmid

    #138560

    Purpose

    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE2-TetO-GFP-mSox2

    Plasmid

    #206374

    Purpose

    Expresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge