We narrowed to 19,051 results for: Tec;

1. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCerulean-LaminB1-10

   Plasmid

   #55380

   Purpose

   Localization: Nuclear Envelope, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 BATF3

   Plasmid

   #67050

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BATF3 (BATF3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pYPQ266

   Plasmid

   #164713

   Purpose

   Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   zCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191155

   Purpose

   Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:DNAJB1.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC012 LwCas13a locus with nontargeting spacer

   Plasmid

   #91904

   Purpose

   LwCas13a locus into pACYC184 with nontargeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with non-targeting spacer
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCMV_Anc689-nCas9

   Plasmid

   #163526

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPyCAG-NE-mKate2-IRES-Hph

   Plasmid

   #183616

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

   Insert

   mKate2
   

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCHECK-2-TGFBR2-wt_3'UTR

    Plasmid

    #31882

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    TGFBR2 wild-type 3'UTR (TGFBR2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAM4951 (pCV0063)

    Plasmid

    #120084

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
    

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREMI-ZA

    Plasmid

    #183736

    Purpose

    Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Sh ble
    

    Use

    Random insertional gene deletion in komagataella …

    Promoter

    TEF1 promoter

    Available Since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Tags

    His-tag

    Expression

    Worm

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. sfGFP-SasG(8G)-mCherry

    Plasmid

    #206950

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (8G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (8G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCosSAD1AprR

    Plasmid

    #190786

    Purpose

    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

    Depositor

    Jan Kormanec

    Article

    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

    Inserts

    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZIKV_Trigger_27B

    Plasmid

    #75008

    Purpose

    Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_27B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196079

    Purpose

    Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196085

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GEV 1

    Plasmid

    #12615

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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