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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 7281 - 7300 of 14359 results

  1. pCAG-TetR-Rad18UBD

    Plasmid
    #119010
    Purpose
    Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression
    Depositor
    Ralf Kuehn
    Publication
    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TetR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    119010-plasmid-map-sequence-id-233203

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  2. pUC19-EAMP

    Plasmid
    #138265
    Purpose
    mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCas9 target-mCherry
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138265-plasmid-map-sequence-id-273624

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  5. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Publication
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR and Gateway Cloning
    Mutation
    Added 3 stop codons to the original sequence; see…
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113745-plasmid-map-sequence-id-260896

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  6. pENTR-R4R3-3xmEGFP-C

    Plasmid
    #113751
    Purpose
    Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Publication
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEGFP-mEGFP-mEGFP
    Use
    CRISPR and Gateway Cloning
    Tags
    3xmEGFP
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113751-plasmid-map-sequence-id-238963

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  7. pENTR-R4R3-3xmRuby-N

    Plasmid
    #113754
    Purpose
    Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Publication
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRuby2-mRuby2-mRuby2
    Use
    CRISPR and Gateway Cloning
    Tags
    3xmRuby2
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113754-plasmid-map-sequence-id-238968

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  15. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Publication
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Expression
    Bacterial
    Promoter
    Xyl/tet promoter that is constitutively active
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

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  17. pGTRm

    Plasmid
    #127197
    Purpose
    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences
    Depositor
    Jordan Green
    Publication
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tRNA-gRNA PCR template
    Use
    Template plasmid for generation of golden gate pc…
    Promoter
    None
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127197-plasmid-map-sequence-id-248423

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Showing: 7281 - 7300 of 14359 results