We narrowed to 17,340 results for: EMB

1. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)- PC-MjMAT

   Plasmid

   #179372

   Purpose

   PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

   Depositor

   Andrea Rentmeister

   Article

   Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

   Insert

   PC-MjMAT
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L147A, I351A

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Hook3 aa 1-552

   Plasmid

   #74614

   Purpose

   Expresses a truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-552 (HOOK3 Human)
   

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECFP-histone H3

   Plasmid

   #179698

   Purpose

   visualization of nucleus/chromosome

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Histone H3 (H3c10 Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU325

   Plasmid

   #167676

   Purpose

   Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-GFP-SF3B6-FLAG

   Plasmid

   #86870

   Purpose

   To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   SF3B6 (SF3B6 Human)
   

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.R267

   Plasmid

   #138179

   Purpose

   Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-SRGAP3-EGFP

    Plasmid

    #168504

    Purpose

    Expression of full length SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3 (SRGAP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Halo-TCAB1 KI sgRNA

    Plasmid

    #207611

    Purpose

    sgRNA for HaloTag insertion into the endogenous TCAB1 locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    CCAAAGTCTTCATACTGCAG
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFPZFN1.4-B2H

    Plasmid

    #26791

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN1
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLJM1 RagA

    Plasmid

    #73031

    Purpose

    Expresses human RagA from a CMV promoter for stable expression in mammalian cells

    Depositor

    David Sabatini

    Article

    Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

    Insert

    rragA (RRAGA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-V5-hTEV

    Plasmid

    #65800

    Purpose

    expresses human codon-optimised TEV protease

    Depositor

    Douglas Green

    Article

    Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

    Insert

    V5-human TEV
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    codon-optimised to express in mammalian cells

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid

    #182009

    Purpose

    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    IgKchL_HA_STf_myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFP.R377

    Plasmid

    #138247

    Purpose

    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-mAV

    Plasmid

    #129472

    Purpose

    Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

    Depositor

    Elizabeth Luna

    Article

    Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

    Insert

    Supervillin, isoform 2 (Svil Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFPZFN2-B2H

    Plasmid

    #26792

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN2
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pJBJZ_003

    Plasmid

    #155358

    Purpose

    Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCDM8 hCD81

    Plasmid

    #11588

    Depositor

    Shoshana Levy

    Article

    Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

    Insert

    CD81 (CD81 Human)
    

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only