We narrowed to 19,050 results for: Tec

1. pC010 LshCas13a locus with nontargeting spacer

   Plasmid

   #91901

   Purpose

   LshCas13a locus into pACYC184 with nontargeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LshCas13a locus with non-targeting spacer
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AKT-APEX2-P2A-mKate2

   Plasmid

   #67654

   Purpose

   Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   AKT-PH-APEX2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. VQR variant

   Plasmid

   #138560

   Purpose

   Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA406

   Plasmid

   #216070

   Purpose

   Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1.1-eGFP

   Plasmid

   #162738

   Purpose

   Fluorescent reporter for CHO cells expression studies

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Insert

   enhanced green fluorescent protein
   

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC240

   Plasmid

   #118895

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and mCherry, clea…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBacDual with LFn + FlaAAA

   Plasmid

   #84867

   Purpose

   Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   FlaAAA
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Mutation

   3 C-terminal leucines have been replaced by alani…

   Promoter

   Polyhedrin

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC175

    Plasmid

    #118890

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. BTK-APEX2-P2A-mKate2

    Plasmid

    #67653

    Purpose

    Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    BTK-PH-APEX2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDARMO_CMVT_3xFLAG-HUWE1_R4187C

    Plasmid

    #187142

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 R4187C

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R4187C

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-RapR-FAK-YM-Y397F

    Plasmid

    #25930

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBL_DK202

    Plasmid

    #156426

    Purpose

    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Tags

    EGFP-His

    Expression

    Mammalian

    Mutation

    aa 42-275

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAP2-beta-actin in modified pEGFP

    Plasmid

    #34839

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LAP2-beta-Actin (ACTB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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