We narrowed to 8,779 results for: chloramphenicol
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Plasmid#167279PurposeCodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only
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pAAVS1-Nst-CAG-DEST
Plasmid#80489PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)ExpressionMammalianAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
Plasmid#172506PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloningDepositorInsertDisCas7-11 bacterial expression vector with golden gate site for guides
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TET
Plasmid#20909PurposepiggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)DepositorInsertGateway Destination Vector
UsepiggybacTagsIRES-bGeoExpressionMammalianAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
ExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD442
Plasmid#153024PurposedCas9, MCP-SoxS(R93A/S101A)DepositorInsertdCas9, MCP-SoxS(R93A/S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsAvailable SinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 HDV (GB1444)
Plasmid#160576PurposeHepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.DepositorInsertHDV
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-DEST
Plasmid#106348PurposeDestination vector for gateway cloning. Developed for Flp-In T-REx system.DepositorTypeEmpty backboneUseGateway CloningExpressionMammalianAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-KPS12-Pman
Plasmid#133832PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECIA14
Plasmid#47051PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tagsDepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsAlkaline Phosphatase (human, placental), Flag, HR…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMS_366
Plasmid#182131Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutationDepositorInsertsretron RNA
ec.86 RT
CspRecT
Mutationincorporated rifampicin-resistance-conferring don…Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway dre SE-zic2a Q
Plasmid#89386PurposeVector for reporter assay of one zebrafish super-enhancer regionDepositorInsertzic2a (zic2a Zebrafish)
Available SinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry
Plasmid#114322PurposeMifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expressionDepositorTypeEmpty backboneUseLentiviralAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTBL2142 MTK3-sfGFP
Plasmid#226661PurposeMTK3 part containing the coding sequence of sfGFPDepositorInsertsfGFP
UseSynthetic BiologyExpressionMammalianPromoternoneAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST exorh:mGreenLantern
Plasmid#195988PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherAvailable SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only