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pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Desmoglein2-mCherry

Plasmid

#36991

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsg2 (DSG2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Blast

Plasmid

#227260

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230989

Purpose

Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-Flag-APH4-Fibcon-icam

Plasmid

#233077

Purpose

Expresses synCAM-APH4-Fibcon-icam

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

APH4-FIBCON-ICAM

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJRA110

Plasmid

#209343

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mStayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKB01_mOrange2

Plasmid

#69102

Purpose

Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mOrange2

Use

Synthetic Biology

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Xenopus APC-myc

Plasmid

#16686

Depositor

Barry Gumbiner

Article

Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

Insert

APC (apc.L Frog)

Use

Xenopus expression

Tags

Myc

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMP4639

Plasmid

#107771

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pME6010

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg61-PDGFR_EF1a_eGFP

Plasmid

#186310

Purpose

Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg61-PDGFR
eGFP

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-Tubbyc

Plasmid

#202677

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505): PAmCherry1 (Tub Mouse)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Article

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pLV-tetO-mCherry

Plasmid

#70273

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of mCherry

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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