We narrowed to 11,022 results for: ENA;

1. pLXI_TRC401 LacZ

   Plasmid

   #111183

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)

   Plasmid

   #198058

   Purpose

   Generation of rodent iPS cells from somatic cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Yamaguchi et al Methods Mol Biol. 2014;1210:143-50. doi:

   Insert

   mOct4, mKlf4, mSox2 (Sox2, Pou5f1, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p21-HA-NeoR

   Plasmid

   #215104

   Purpose

   Expresses p21-HA in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-Beta4-spectrin [N393/2R]

   Plasmid

   #177541

   Purpose

   Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. IP3 receptor, type 1 scFv [L24/1]

   Plasmid

   #206755

   Purpose

   Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/1 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. peSCBE3-NG-HF1-Hypa

   Plasmid

   #218166

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

   Plasmid

   #185706

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   Cas9
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FFSS-Cyclin D1-NeoR

   Plasmid

   #215087

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p21-HA(dPCNA)-NeoR

   Plasmid

   #215108

   Purpose

   Expresses p21-HA(dPCNA) in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #187324

    Purpose

    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-Nav1.4 Na+ channel [N255/38]

    Plasmid

    #190306

    Purpose

    Mammalian Expression Plasmid of anti-Nav1.4 Na+ channel (Rat). Derived from hybridoma N255/38.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Nav1.4 Na+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Scn4a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GluN2B/NR2B glutamate receptor scFv [N59/36]

    Plasmid

    #182093

    Purpose

    Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/36.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-TrpC6 [N490/24R]

    Plasmid

    #211590

    Purpose

    Mammalian Expression Plasmid of anti-TrpC6 (Human) IgG2a R-mAb. Derived from hybridoma N490/24.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-TrpC6 (Homo sapiens) recombinant mouse monoclonal antibody. (TRPC6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-NLS-HA-TurboID-PCNA

    Plasmid

    #215074

    Purpose

    Expresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    NLS-HA-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-tdTHC

    Plasmid

    #112621

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid

    #217800

    Purpose

    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid

    #217805

    Purpose

    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-VGlut3 [N34/34R-2b]

    Plasmid

    #219476

    Purpose

    Mammalian Expression Plasmid of anti-VGlut3 neurotransmitter transporter (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N34/34.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-VGlut3 neurotransmitter transporter (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc17a8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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