We narrowed to 7,159 results for: GFP expression plasmids
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Plasmid#103979PurposePlasmid encoding mEGFPDepositorInsertmEGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_NOT_i1
Plasmid#231416PurposeThis NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.DepositorInsertsdCas9
GFP
UseSynthetic BiologyAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_81
Plasmid#60073PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_47
Plasmid#60071PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_84
Plasmid#60076PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_83
Plasmid#60075PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_82
Plasmid#60074PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_80
Plasmid#60072PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRExpressionMammalianPromoterU6Available SinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA
Plasmid#109318PurposePlasmid for liver-specific expression of EmGFP with a gRNA against SaCas9DepositorInsertSaCas9 RuvC gRNA
UseAAV and CRISPRExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Cre
Plasmid#175381Purposeexpresses GFP-Cre under the control of a human synapsin promoterDepositorInsertGFP-Cre recombinase
UseAAVTagsCre is fused to GFP. GFP is fused to additional n…Promoterhuman synapsinAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-MCS-noATG-GFP
Plasmid#223181PurposeA multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid.DepositorTypeEmpty backboneUseAAVAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Gluc-NHP.RMA-IRES-EGFP
Plasmid#227887PurposeExpresses Gluc-NHP.RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-NHP.RMA and EGFP for monitoring Cre-expressing neuronal populations.DepositorInsertsGaussia luciferase fused to macaque Fc
IRES-EGFP
UseAAV, Cre/Lox, and LuciferaseTagsGluc and IgG1 FcExpressionMammalianAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP
Plasmid#172221PurposeCas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.DepositorTypeEmpty backboneUseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFA104.PI.eGFP.WPRE.bGH
Plasmid#100896PurposeAAV expression of EGFP in astrocytesDepositorHas ServiceAAV5InsertEGFP
UseAAVExpressionMammalianPromoterGFA104 (GFAP promoter)Available SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
Tags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP2-EGFP
Plasmid#86073PurposeA mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.DepositorAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spGFP
Plasmid#225486PurposeTet-on lentiviral vector for controlled expression of secretable and permeable EGFPDepositorInsertIgκ leader and TAT peptide
UseLentiviralTagsEGFPExpressionMammalianPromoterHuman ubiquitin CAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-eGFP
Plasmid#192589PurposeAAV-vector for fluorescent labeling of noradrenergic neuronsDepositorInsertEGFP
UseAAVExpressionMammalianPromoterPRSX8Available SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
ExpressionMammalianAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C+F-GFP
Plasmid#209699PurposeExpresses GFP dependent on the combined activity of CRE- and FLP-recombinasesDepositorInsertGFP
UseAAVPromoterCBAAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-GFP
Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVExpressionMammalianPromoterSynAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-PERK(LD)-EGFP
Plasmid#225680PurposeUsed to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-Nb(VHHGFP4)
Plasmid#198412PurposeExpress mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-GFP
Plasmid#178580PurposeExpression of GFP under the human GFAP promoterDepositorInsertGFP
UseAAVExpressionMammalianAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
TagsHis6 and SUMOExpressionBacterialPromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-alpha2-chimaerin
Plasmid#59316PurposeExpression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)DepositorInsertalpha2-chimaerin
TagsGFPExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)
Plasmid#185913PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)
Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV-hTau-T2A-EGFP-WPRE3-BGHpA
Plasmid#240284PurposeThe plasmid will package wild-type human Tau (hMAPT) as a T2A fusion with EGFP from a CMV promoterDepositorAvailable SinceOct. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK
Plasmid#139279PurposeB6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseCre/Lox and Mouse TargetingTagsC-terminal fusion of T2A-sfGFP-iCre-ERT2ExpressionMammalianAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eA3A-BE5-(NG)-PX458-EGFP
Plasmid#229687PurposeCRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsEGFPExpressionMammalianAvailable SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUW-[EF1α-CD19 4-1BB-eGFP]
Plasmid#218342PurposeLentiviral transfer plasmids for mammalian expression CD19 CARDepositorInsertCD19 CAR mammalian expression cassette
UseLentiviralExpressionMammalianAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synmiR-10-mRuby-Pro-EGFP-8×8
Plasmid#218260PurposeExpresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuitDepositorInsertsynPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×8
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synmiR-10-mRuby-Pro-EGFP-8×9
Plasmid#218261PurposeExpresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuitDepositorInsertsynPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×9
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synmiR-L-mRuby-Pro-EGFP-4×17
Plasmid#218262PurposeExpresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuitDepositorInsertsynPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×17
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synmiR-4-mRuby-Pro-EGFP-4×8
Plasmid#218237PurposeExpresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuitDepositorInsertsynPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_4×8
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synmiR-4-mRuby-Pro-EGFP-8×8
Plasmid#218238PurposeExpresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuitDepositorInsertsynPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_8×8
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only