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Plasmid#121069PurposeNegative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNADepositorInsertGPHN shRNA (Gphn Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-Epsin 2
Plasmid#22241DepositorInsertEpsin 2 (EPN2 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xBtg-X
Plasmid#24964DepositorInsertBtg-X (MGC76328.S Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His
Plasmid#207665PurposeExpresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tagDepositorInsertFucosidase E1_10125
UseTagsHis-tagExpressionBacterialMutationamino acids 34-584 were usedPromoterT7 promoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_TetOn_Hu_MAD2(WT)
Plasmid#136347PurposeLentiviral inducible expression (TetOn) of human MAD2DepositorInsertMAD2L1 (MAD2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594G
Plasmid#131719PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationD594GPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Septin 11 isoform III
Plasmid#120319PurposeSeptin 11 isoform III mammalian expressionDepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-EPSIN 1
Plasmid#22225DepositorInsertEpsin 1 (Epn1 Rat)
UseTagsXpressExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
peft-3::cas-9::tbb-2 3'UTR
Plasmid#48960Purposethis plasmid contains the codon optimized gene encoding Cas9 for C. elegans.DepositorInsertcas9
UseCRISPRTagsExpressionMutationPromotereef-1A.1 (eft-3)Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-N FLAG
Plasmid#12541DepositorInsertN FLAG
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
Plasmid#182547PurposeExpresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificityDepositorInsertH5_Vietnam_1203_04_Y161A
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationY161A mutant that confers exclusive NeuAc to NeuG…PromoterCMVAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu MAD2(WT)
Plasmid#136343PurposeHuman MAD2 cDNA in Gateway pENTRDepositorInsertMAD2L1 (MAD2L1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro- TRF2
Plasmid#18009DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsExpressionMammalianMutationaa2-500PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK-E3mut
Plasmid#29691DepositorInsertGFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
UseTagsFlag and GFPExpressionMammalianMutationrecognition sites only, and E3 ligase site mutatedPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
Myopathy Loop Mutant Nuclear Myosin I YFP
Plasmid#60243PurposeExpresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activityDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationDeleted I339, I340, G343, E344, E345,L346PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX335-NQL002-WAPL-sgRNA1
Plasmid#175550PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.DepositorInsertspCas9-nickase and sgRNA against mouse WAPL STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX335-NQL003-WAPL-sgRNA2
Plasmid#175551PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.DepositorInsertspCas9-nickase and sgRNA against mouse WAPL STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
delta-EGFP
Plasmid#119223PurposeGABAA receptor expression (delta subunit with EGFP tag at the C-terminus)DepositorInsertGABRD (Gabrd Rat)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p16-1 hTRF2
Plasmid#12299DepositorInserthTRF2 (TERF2 Human)
UseTagsExpressionBacterialMutationA434 deleted; There is a stop codon in the 5'…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBluescript SK - hTRF1
Plasmid#12306DepositorInserthTRF1 (TERF1P3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only