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Plasmid#186633Purposecontains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pJMH021
Plasmid#186634Purposecontains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH022
Plasmid#186635Purposecontains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH036
Plasmid#186637PurposeaadA.co between homology arms for NSIDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH043
Plasmid#186638PurposeaadA.co between homology arms for oscillinDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-MAFB-sg
Plasmid#194720Purposepx458 with guide RNA that target hMAFBDepositorArticleInsertCas9 (MAFB Streptococcus pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_mdHUR-Halo
Plasmid#175312PurposeProduction of HaloTag fusion proteinDepositorInsertHUR
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS33
Plasmid#171202Purposebacterial experssion of a GST-tagged MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTagsGSTExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB252
Plasmid#170035PurposeEncodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K6L9
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAP21
Plasmid#171207Purposebacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags8xHISExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW004
Plasmid#131805PurposeVector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'DepositorArticleInsertZea mays FNR and Z. mays SIR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG09-OR2-OR1-Pr-sigma54
Plasmid#102466PurposeExpresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertsigma 54 (rpoN )
UseTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG69-KcsA-Kv1.3-deGFP
Plasmid#102458PurposeExpresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.DepositorArticleInsertsKcsA-Kv1.3
deGFP
UseTagsHis tagExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKB254
Plasmid#170037PurposeEncodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
H6L9
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-mCherry-Hygro
Plasmid#210852PurposeLentiviral expression vector encoding FIP200-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-Linker-GFP
Plasmid#211734PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist GFP-Linker-VDAC1
Plasmid#211735PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tTA
Plasmid#149363PurposeAAV-mediated and Cre-dependent expression of tTA under the CAG promoter.DepositorInserttTA2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-dtA
Plasmid#232236PurposeExpresses diphtheria toxin A subunit in Cre-positive cells.DepositorInsertDiphtheria toxin A subunit
UseAAVTagsExpressionMammalianMutationPromoterCAAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only