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MSP3.5-bio-His

Plasmid

#50803

Purpose

Expresses enzymatically monobiotinylated full length MSP3.5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP3.5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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MSP8-bio-His

Plasmid

#50807

Purpose

Expresses enzymatically monobiotinylated full length MSP8 ectodomain

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP8

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-zEPLIN

Plasmid

#40957

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN (lima1 Zebrafish)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
4xHRE_CMV53-sfGFP-CMV_dsRed Exp

Plasmid

#117402

Purpose

sfGFP expressed from hypoxia-inducible CMV53 promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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4xHRE_miniTK-sfGFP-CMV_dsRed Exp

Plasmid

#117404

Purpose

sfGFP expressed from hypoxia-inducible miniTK promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-Esp3I

Plasmid

#81226

Purpose

Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

Esp31-Neo-Esp31 deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAG148 SGCD 3'UTR

Plasmid

#12006

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N13 3'UTR (SGCD Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG192 PIK3CG 3'UTR

Plasmid

#12019

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N23 3'UTR (PIK3CG Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L5-L4

Plasmid

#62102

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-L4

Plasmid

#62112

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCALNL_Ch10

Plasmid

#81224

Purpose

pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAG216 NEK6 3'UTR mut

Plasmid

#12046

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N17 3'UTR mut (NEK6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L5-L2

Plasmid

#62103

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAG178 RGS7 3'UTR mut

Plasmid

#12036

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N11 3'UTR mut (RGS7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-B6

Plasmid

#31710

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-19b

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only