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pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJH4723

Plasmid

#178899

Purpose

Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJDC124

Plasmid

#157877

Purpose

mCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

mCherry

Expression

Bacterial

Promoter

phsp60

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Tags

Linker and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-ro2GFP

Plasmid

#82370

Purpose

Expression of redox sensitive GFP in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr

Plasmid

#221109

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-UBC9

Plasmid

#228949

Purpose

express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

Depositor

Marcin Suskiewicz

Insert

UBC9 (UBE2I Human)

Tags

His tag and thrombine cleavage site : MGHHHHHHSSG…

Expression

Bacterial

Promoter

T7-lac

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pWS5396-CRISPRai-KanR

Plasmid

#182835

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc

Plasmid

#84656

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only