We narrowed to 14,728 results for: EGF

1. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Plasmid

   #179115

   Purpose

   Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

   Plasmid

   #190112

   Purpose

   AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Inserts

   MMLV-RT(dRH)
   U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
   

   Use

   AAV

   Tags

   bpNLS and bpNLS-P2A-eGFP

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   EFS and U6, H1

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

   Plasmid

   #208377

   Purpose

   Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132397

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1058A

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

   Plasmid

   #174529

   Purpose

   Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Inserts

   PylS
   PylT
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

   Plasmid

   #191159

   Purpose

   Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:EGFP.s2:mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

    Plasmid

    #127332

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

    Plasmid

    #149722

    Purpose

    Lentiviral expression of crRNA with GFP and a neomycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid

    #135955

    Purpose

    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Prime editor v2 (PE2) N-terminal
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132410

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dEsCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R295A; H300A; R849A; H854A

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid

    #123363

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    EGFP-NLS
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132411

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRfxCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R239A; H244A; R858A; H863A

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid

    #208289

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA; mutations in iSpyMac

    Promoter

    CMV and T7

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

    Plasmid

    #208378

    Purpose

    Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge