We narrowed to 19,050 results for: Tec

1. pCellFree_G03 BTF3

   Plasmid

   #67059

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BTF3 (BTF3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 SMAD2

   Plasmid

   #67095

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-LmoCascade-IL1RNcrRNA

   Plasmid

   #126493

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   Lmo IL1RN cr03
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-LmoCascade-HBE1crRNA

   Plasmid

   #126494

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   Lmo HBE1 cr06
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 TYK2

   Plasmid

   #67133

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TYK2 (TYK2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   A435S

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Zebrafish-gRNA-0002

   Plasmid

   #42242

   Purpose

   gRNA targeted to zebrafish gene drd3

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-drd3 (drd3 Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

7. TAL3071

   Plasmid

   #41245

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-ets1a-Right (ets1 Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TAL3070

   Plasmid

   #41244

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-ets1a-Left (ets1 Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pOCC177

   Plasmid

   #118886

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease and SNAP tag…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC194

    Plasmid

    #118882

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    SNAP tag, cleavable with TEV protease, HIS6, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC210

    Plasmid

    #118896

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and SNAP tag, cle…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC231

    Plasmid

    #118885

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mCitrine…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB3037(XI-5 MarkerFree)

    Plasmid

    #73277

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPK1E-1+t-gfp

    Plasmid

    #48131

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPTXGARE

    Plasmid

    #68542

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

    Promoter

    OPTX promoter with GARE

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiCHECK-2-Rhoc-m_3'UTR

    Plasmid

    #31885

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    Rhoc mutant 3'UTR (RHOC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    miR-302b, 372 7-mer seed match mutated from AGCAC…

    Promoter

    SV40

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. TAL3403

    Plasmid

    #47122

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-right (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. TAL3402

    Plasmid

    #47121

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-left (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pNAS1b split mCherry NEDD4 WW2

    Plasmid

    #111882

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Tags

    Split mCherry system: NEDD4 WW2 fused to C-termin…

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-BE3-2X

    Plasmid

    #110835

    Purpose

    CMV expression vector for BE3-2X construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-2X
    

    Expression

    Mammalian

    Mutation

    2 tandem NLS sequences in the XTEN linker

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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