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pUC118-FLIP-FlpE-Puro

Plasmid

#84539

Purpose

a mock vector for generating FLIP-FlpE targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP-FlpE - Puro

Use

Cre/Lox and Mouse Targeting

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-hUBP43

Plasmid

#12455

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (USP18 Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
PAmCherry1-C1-P4Mx2

Plasmid

#202681

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CMV:GFP

Plasmid

#68485

Purpose

Retrovirus expressing GFP driven by CMV promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Lyric-mCherry

Plasmid

#226563

Purpose

Encodes Lyric protein labeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo-mAID

Plasmid

#112852

Purpose

General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Halo-mAID

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMK486 (Hygro-P2A-2mAID)

Plasmid

#214398

Purpose

2mAID for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-2mAID

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-PGK-Puro

Plasmid

#227264

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-SINTBAD

Plasmid

#210209

Purpose

For mammalian expression of SINTBAD (Internal ref. SMC2249)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBKBP1 (TBKBP1 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-DLL1

Plasmid

#205192

Purpose

This vector encodes of a synCAM (DLL1) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM DLL1 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-ZnT1-WT

Plasmid

#82340

Purpose

Expresses FLAG-tagged ZnT1-WT in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

ZnT1-WT (SLC30A1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pMRS-dCas9

Plasmid

#220193

Purpose

mini-Tn7 dCas9 expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDG04163

Plasmid

#216373

Purpose

xhNup214-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup214-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-scSUMO

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHRSin_hCD3δ-GFP2

Plasmid

#187355

Purpose

Lentiviral expression of truncated hCD3 delta-GFP2

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

Truncated hCD3 delta, and GFP2

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Traptavidin-E6

Plasmid

#59549

Purpose

pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

Depositor

Mark Howarth

Article

Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

Insert

Core Traptavidin-E6

Tags

E6 tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only