We narrowed to 59,446 results for: cloning

1. pDONR223 NRF2

   Plasmid

   #118708

   Purpose

   entry clone for NRF2

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   NRF2
   

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Dyskerin

   Plasmid

   #207577

   Purpose

   N-terminal mCherry-tag using SalI/EcoRI

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/N-mChe

   Plasmid

   #183159

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. bdSUMO-pHD118-GFP-HiBit

   Plasmid

   #171015

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHD118-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO GFP DNAJB1

   Plasmid

   #19495

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB1 (DNAJB1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS hShh (CT#401)

   Plasmid

   #13996

   Depositor

   Cliff Tabin

   Article

   Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

   Insert

   Shh (SHH Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. bdSUMO-pHlip-GFP-HiBit

   Plasmid

   #171016

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHlip-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK1520_puroR

   Plasmid

   #173901

   Purpose

   SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

   Depositor

   Ronald Cohn

   Article

   Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV/U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO V5 HSPA2

   Plasmid

   #19512

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 2 (HSPA2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid

    #175509

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQStrep2-PREI3

    Plasmid

    #31592

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    preimplantation protein 3 (MOB4 Human)
    

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENT-L1-Kozak-lacZ-stop-L2

    Plasmid

    #186359

    Purpose

    Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli, Synthetic)
    

    Use

    Expression of lacz reporter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV Flag PAI-RBP

    Plasmid

    #45452

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pNP1 sfGFP toehold switch

    Plasmid

    #107355

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold 7 + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLC-242

    Plasmid

    #73189

    Purpose

    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    

    Expression

    Bacterial

    Promoter

    chloramphenicol promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19/mChe-C

    Plasmid

    #183160

    Purpose

    Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/eCFP-C

    Plasmid

    #183156

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYL322d1/N-eGFP

    Plasmid

    #183151

    Purpose

    Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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