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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,379 results for: Cre

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Showing: 7441 - 7460 of 14379 results

  4. pYPQAT27

    Plasmid
    #223399
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223399-plasmid-map-sequence-id-452308

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  6. pDEST26-MSRA-C-HA

    Plasmid
    #195177
    Purpose
    Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Stephanie Roessler
    Insert
    MSRA (MSRA Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195177-plasmid-map-sequence-id-423103

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  8. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Publication
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVvanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  12. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Publication
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  15. HTR2A-DuET

    Plasmid
    #213321
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2A (HTR2A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213321-plasmid-map-sequence-id-430857

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  16. GPR84-DuET

    Plasmid
    #213292
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR84 (GPR84 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213292-plasmid-map-sequence-id-430837

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  20. pSTAP-seq_human-4xUAS

    Plasmid
    #125150
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells
    Depositor
    Alexander Stark
    Publication
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    125150-plasmid-map-sequence-id-254778

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Showing: 7441 - 7460 of 14379 results