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pVRC-N-Venus-V150A-CC-Di

Plasmid

#190707

Purpose

Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-CC-Di

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A

Plasmid

#190708

Purpose

Control plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB7_9-ND51_v8_X1

Plasmid

#191511

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE 9-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC207-MXMT

Plasmid

#182730

Purpose

Transient expression of KOC207-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC207

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pL6_3xG34-DCAF8L2_X1

Plasmid

#191518

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-SpDcp1opt_AD

Plasmid

#148538

Purpose

Bacterial Expression of SpDcp1opt

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp1opt (dcp1 Fission Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-Nluc

Plasmid

#192659

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pL5N_LT32-HBB_X1

Plasmid

#191520

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE LT32-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL7_3xG35-EMILIN2_X1

Plasmid

#191517

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG35-EMILIN2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB6_R13-ND51_v8_X1

Plasmid

#191512

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE R13-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ntag

Plasmid

#192096

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ntag

Plasmid

#192094

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ctag

Plasmid

#192099

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ntag

Plasmid

#192100

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLTR-DuvSAF

Plasmid

#190914

Purpose

Pseudotyping of lentiviral vectors

Depositor

Jakob Reiser

Article

Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

Insert

Lyssavirus duvenhage antigenic glycoprotein

Expression

Mammalian

Promoter

HIV-1 LTR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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