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pcDNA3-rB50

Plasmid

#18957

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican N-terminus (Bcan Rat)

Expression

Mammalian

Mutation

Brevican N-terminus, aminoacids 1-395

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
SUN2delCC

Plasmid

#130836

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of coiled coil domain

Promoter

CaMV 35S

Availability

Academic Institutions and Nonprofits only

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SUN2delSUNec

Plasmid

#130847

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway Cloning

Tags

mCherry-FLAG-HA

Mutation

Deletion of SUN domain

Availability

Academic Institutions and Nonprofits only

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SUN2delCCec

Plasmid

#130848

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway Cloning

Tags

mCherry-FLAG-HA

Mutation

Deletion of coiled coil domain

Availability

Academic Institutions and Nonprofits only

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Protrudin-FFAT

Plasmid

#253737

Purpose

Abolish VAP binding via FFAT motif disruption

Depositor

Evan Reid

Article

Kleniuk et al Neurobiol Dis. 2026 Jan;218:107231. doi:

Insert

Protrudin with mutant FFAT

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Protrudin-FYVE

Plasmid

#253738

Purpose

Abolish binding to endosomal phosphoinositides

Depositor

Evan Reid

Article

Kleniuk et al Neurobiol Dis. 2026 Jan;218:107231. doi:

Insert

Protrudin with mutant FYVE

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Protrudin-ΔCC

Plasmid

#253739

Purpose

Remove kinesin-binding coiled-coil domain

Depositor

Evan Reid

Article

Kleniuk et al Neurobiol Dis. 2026 Jan;218:107231. doi:

Insert

Protrudin with mutant CC

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-DogCatcher

Plasmid

#171929

Purpose

Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

Cys-DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLCa

Plasmid

#196912

Purpose

Visualization of clathrin-coated pit formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

Insert

Clathrin-Coated light chain

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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