Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,244 results for: BASE

Clear keywords
Showing: 7481 - 7500 of 13244 results

  1. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

    Enlarge

  3. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human Synapsin 1
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105983-plasmid-map-sequence-id-235283

    Enlarge

  4. qTAG-C-Solo-mStayGold-CLTC

    Plasmid
    #227313
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227313-plasmid-map-sequence-id-451694

    Enlarge

  7. pNOC_hfnCas12a_NR1_NR2

    Plasmid
    #176252
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    176252-plasmid-map-sequence-id-346964

    Enlarge

  16. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121722-plasmid-map-sequence-id-237078

    Enlarge

Showing: 7481 - 7500 of 13244 results