We narrowed to 165,853 results for: Mal;

1. pJHG003

   Plasmid

   #126454

   Purpose

   Expresses Cas9-XTEN-dTdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   Added D343E and D345E to inactivate TdT.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK296 (DHC1-mAID Neo)

   Plasmid

   #140541

   Purpose

   DHC1 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-S (B.1.351 lineage / South African variant)

   Plasmid

   #169849

   Purpose

   Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag

   Depositor

   Erik Procko

   Article

   Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

   Insert

   Protein S
   

   Tags

   HA leader and c-myc tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

   Plasmid

   #73266

   Purpose

   Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Tags

   His/V5

   Expression

   Mammalian

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP C1-Eps8 ∆cap/∆bund

   Plasmid

   #74891

   Purpose

   mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

   Insert

   Eps8 (Eps8 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   V689D, L693D, L757A, K759A

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pChuk

   Plasmid

   #87033

   Purpose

   express IKKalpha in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   IKKalpha (Chuk Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-DDX11-Flag-KAE

   Plasmid

   #120728

   Purpose

   Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

   Depositor

   Francesca Pisani

   Article

   Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

   Insert

   DDX11 KAE mutant (DDX11 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Substitution of E201 and Y202 with K and A

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-DDX11-Flag-KAK

    Plasmid

    #120729

    Purpose

    Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAK mutant (DDX11 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201, Y202 and E203 with K, A and…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NET23 pEGFP-N2 (1174)

    Plasmid

    #62037

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NET23 (STING1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. STT3A pmRFP (663)

    Plasmid

    #61997

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. mHDAC7-Nterm (23-504) pEF6-V5

    Plasmid

    #73270

    Purpose

    Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Tags

    His/V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 505-938

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    Pgk and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX304-BCL-XL-F97W

    Plasmid

    #203572

    Purpose

    Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 97 to Tryptophan for partia…

    Promoter

    CMV

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBpuro-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209919

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbleo-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209920

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBbsr2-miRFP703-eDHFR(69K6)-cRaf

    Plasmid

    #209921

    Purpose

    Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-BCL-XL-499R

    Plasmid

    #203574

    Purpose

    Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5- tagged

    Expression

    Mammalian

    Mutation

    silent point mutations introduced to make it resi…

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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