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LT2_34_EthPro_X2site

Plasmid

#194430

Purpose

Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

EthPro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCA31-pCS2-CMV-stdPCP-p65-HSF1

Plasmid

#199462

Purpose

Expression of stdPCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA30-pCS2-CMV-stdMCP-p65-HSF1

Plasmid

#199461

Purpose

Expression of stdMCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pTA2EGFP

Plasmid

#193756

Purpose

Expresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3RBSC1cI

Plasmid

#193773

Purpose

Expresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX*C3E2-Crimson

Plasmid

#193754

Purpose

Expresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLUX*

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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PRC3MKO2

Plasmid

#193757

Purpose

Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mKO2

Expression

Bacterial

Promoter

PR

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLC3RBSC2cI

Plasmid

#193775

Purpose

Expresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Expression

Bacterial

Promoter

PLLacO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJA2LUXR(F)

Plasmid

#193755

Purpose

Expresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

LuxR

Expression

Bacterial

Promoter

BBa_J23102

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CcuCas9

Plasmid

#192127

Purpose

Expresses CcuCas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

CcuCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l AEC

Plasmid

#196473

Purpose

Protein expression of SpyTag-ELP-CarHC

Depositor

Fei Sun

Article

Yang et al Matter (2020) 2, 233–249

Insert

AEC

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp2Cas9

Plasmid

#192126

Purpose

Expresses Hsp2Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1Cas9

Plasmid

#192125

Purpose

Expresses Hsp1Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuxD

Plasmid

#188308

Purpose

Expression of LuxD via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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InterTag

Plasmid

#186910

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-PDGFR

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-420

Plasmid

#192993

Purpose

Plasmid that encodes for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-Luz

Plasmid

#188304

Purpose

Expression of Luz via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luz

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuzGFPHis

Plasmid

#188315

Purpose

Expression of Luz-eGFP via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Luz-GFP fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-Fre

Plasmid

#188310

Purpose

Expression of Fre via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

fre

Use

Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuxC

Plasmid

#188307

Purpose

Expression of LuxC via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-H3H

Plasmid

#188303

Purpose

Expression of H3H via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

h3h

Use

Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-HisGFPLuz

Plasmid

#188314

Purpose

Expression of eGFP-Luz via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

GFP-Luz fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuxE

Plasmid

#188309

Purpose

Expression of LuxE via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxE

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2At(2nd)

Plasmid

#183085

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2At(2nd)

Plasmid

#183086

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PET28a-cpLumiLuc-amylase sensor

Plasmid

#186293

Purpose

Expression alpha-amylase sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-amylase sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PET28a-cpLumiLuc-HSA sensor

Plasmid

#186294

Purpose

Expression Human serum albumin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-HSA sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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