We narrowed to 59,446 results for: cloning

1. KTK_001

   Plasmid

   #180517

   Purpose

   Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

   Plasmid

   #185711

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed I770 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xFLAG-CMV-zDicer

   Plasmid

   #171539

   Purpose

   Encodes Zebrafish Dicer protein

   Depositor

   Yan Zeng

   Article

   Li et al Biochem Biophys Res Commun. 2019 Jun 18;

   Insert

   dicer1(MK642699) (dicer1 Zebrafish)
   

   Expression

   Mammalian

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0.062

   Plasmid

   #119651

   Purpose

   Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   Bba_B0011
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB1_34_RPS25Att

   Plasmid

   #98526

   Purpose

   BB1 with insert: RPS25Att

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   RPS25Att
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. KTK_247

   Plasmid

   #180529

   Purpose

   Entry vector containing pTac promoter

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   pTac promoter
   

   Expression

   Bacterial

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO HIS DNAJA4

   Plasmid

   #19548

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA0.001

   Plasmid

   #119557

   Purpose

   Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. kank2_pJet1.2

   Plasmid

   #121980

   Purpose

   for generating kank2 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank2 (LOC571549 Zebrafish)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA6/ERGIC2-WT

    Plasmid

    #51479

    Purpose

    Expresses ERGIC2-WT in mammalian cells

    Depositor

    Simon Kwok

    Article

    Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

    Insert

    ERGIC2-WT (ERGIC2 Human)
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ914

    Plasmid

    #44489

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBP-ORF

    Plasmid

    #72949

    Purpose

    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - ORFs
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNP1 OR sfGFP

    Plasmid

    #107364

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    OR gate + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB1_23_eGFP

    Plasmid

    #98518

    Purpose

    BB1 with insert: CDS eGFP

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    eGFP
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPi

    Plasmid

    #127548

    Purpose

    Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment

    Depositor

    Luis Vidali

    Article

    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

    Insert

    Adenine Phosphorybosyltransferase transcript segment
    

    Use

    RNAi; Empty control

    Promoter

    Ubiquitin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Act5C-pDONR221

    Plasmid

    #124242

    Purpose

    Gateway entry clone of Drosophila Actin5C cDNA.

    Depositor

    Peter Vilmos

    Insert

    Drosophila Actin 5C (Act5C Fly)
    

    Expression

    Insect

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3aK_14*

    Plasmid

    #98529

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. BB1_12_pLAT1

    Plasmid

    #98509

    Purpose

    BB1 with insert: promoter PLAT1

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    LAT1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB1_12_pPFK300

    Plasmid

    #98510

    Purpose

    BB1 with insert: promoter PPpPfk

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    PpPfk promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge