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pDONR223-ZNF331

Plasmid

#88442

Purpose

Donor Vector containing ZNF331 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF331 (ZNF331 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF232

Plasmid

#88305

Purpose

Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF232 (ZNF232 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF442

Plasmid

#101455

Purpose

Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF442 (ZNF442 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF366

Plasmid

#88019

Purpose

Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF366 (ZNF366 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF175

Plasmid

#88551

Purpose

Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF175 (ZNF175 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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API5_pLX307

Plasmid

#98315

Purpose

Lentiviral expression of API5

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

API5 (API5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RVR

Plasmid

#108863

Purpose

FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R, K629R and N633R, Cpf1 is rice codon optimi…

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B-G120A-GFP

Plasmid

#123108

Purpose

Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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MED9_pLX307

Plasmid

#98352

Purpose

Lentiviral expression of MED9

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED9 (MED9 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-FDX1

Plasmid

#251696

Purpose

Gateway-adapted FDX1 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

FDX1 ferredoxin 1 (FDX1 Human)

Use

Gateway donor vector

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-BCL2

Plasmid

#251695

Purpose

Gateway-adapted BCL2 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

BCL2 BCL2 apoptosis regulator (BCL2 Human)

Use

Gateway donor vector

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG NEDD8

Plasmid

#250151

Purpose

Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG NEDD8

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K69R_K709R POLH (4KR)

Plasmid

#246408

Purpose

Expresses K682R_K686R_K694R_K709R POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682R, K686R, K694R, K709R

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682A_K686A_K694A_K709A (4KA) POLH_deltaGG Ubiquitin

Plasmid

#246409

Purpose

Expresses GFP-tagged K682A_K686A_K694A_K709A POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG Ubiquitin

Plasmid

#246410

Purpose

Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_ WT POLH_deltaGG Ubiquitin

Plasmid

#246411

Purpose

Expresses HA-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH_deltaGG NEDD8

Plasmid

#246412

Purpose

Expresses HA-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_L704A_F707A_F708A (PIP2*) POLH

Plasmid

#246283

Purpose

Expresses L704A_F707A_F708A POLH with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH L704A_F707A_F708A. Coding sequence has been …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_NLS~WT POLH

Plasmid

#246405

Purpose

Expresses WT POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS~FLAG

Expression

Mammalian

Mutation

The coding sequence for WT POLH has been optimise…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG NEDD8

Plasmid

#221870

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG NEDD8

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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