We narrowed to 14,099 results for: cas9

1. Sniper2P

   Plasmid

   #193857

   Purpose

   Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-dTLN1 intron

   Plasmid

   #176224

   Purpose

   A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-dTLN1 exon

   Plasmid

   #176033

   Purpose

   A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-v2-ALDH1A3gRNA2

   Plasmid

   #189747

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-v2-ALDH1A3gRNA1

   Plasmid

   #189746

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168243

   Purpose

   "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-DN
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168244

   Purpose

   "Rescue Rac-CA expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-CA
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PEA1-Nuclease

   Plasmid

   #176528

   Purpose

   Delivers all prime editing nuclease components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-U6-UL29-U3-UL8

   Plasmid

   #166685

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA (UL29, UL8 HSV-1)
   

   Use

   Lentiviral

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

    Plasmid

    #173891

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; gcry1: BFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

    Plasmid

    #160609

    Purpose

    TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2-VP64:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-EN1680_Sororin-Cterm

    Plasmid

    #156451

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, SORORIN (Cdca5 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKO_PDPR_exon_deletion_2

    Plasmid

    #155066

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO_SFRS7_exon_deletion_1

    Plasmid

    #155069

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKO_SFRS7_exon_deletion_2

    Plasmid

    #155070

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCHKO_MDM4_exon_deletion_1

    Plasmid

    #155073

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_MDM4_exon_deletion_2

    Plasmid

    #155074

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCHKO_MDM4_exon_deletion_4

    Plasmid

    #155076

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_HPRT1_exon3_1_As

    Plasmid

    #155057

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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