We narrowed to 59,446 results for: cloning

1. BB1_12_pDAS2

   Plasmid

   #98513

   Purpose

   BB1 with insert: promoter PDAS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   DAS2 promoter
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MCV-HF LTS220A/S239A

   Plasmid

   #112191

   Purpose

   Merkel cell polyomavirus molecular clone

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCV-HF LTS220A/S239A
   

   Mutation

   T to G mutations at 3313 (nt) and 3370 (nt) of fu…

   Promoter

   None from vector. Insert contains native promoter…

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB1_12_pRPL2A

   Plasmid

   #98508

   Purpose

   BB1 with insert: promoter PRPL2A

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   RPL2A promoter
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. kank4_pJet1.2

   Plasmid

   #121982

   Purpose

   for generating kank4 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank4 (kank4 Zebrafish)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Rae1-pDONR221

   Plasmid

   #124240

   Purpose

   Gateway entry clone of Drosophila Rae1 cDNA.

   Depositor

   Peter Vilmos

   Article

   Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

   Insert

   Drosophila Rae1 (Rae1 Fly)
   

   Expression

   Insect

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_103

   Plasmid

   #133460

   Purpose

   H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BB1_12_pDAS1

   Plasmid

   #98515

   Purpose

   BB1 with insert: promoter PDAS1

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   DAS1 promoter
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNP1 AND gate sfGFP

   Plasmid

   #107361

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   AND gate + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCK025

    Plasmid

    #105415

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain and regulatory sequences (estrogen receptor)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BB3rN_AH

    Plasmid

    #98556

    Purpose

    empty BB3 for insertion and overexpression of 7 genes in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human NCKX3 (pcDNA3.1+)

    Plasmid

    #75205

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (SLC24A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KHBD00600

    Plasmid

    #39679

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11798 (chn Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5/FRT/TO HIS DNAJB7

    Plasmid

    #19557

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLminP_Luc2P_RE56

    Plasmid

    #247034

    Purpose

    Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NR3C1 (GR)

    Available Since

    Nov. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLminP_Luc2P_RE58

    Plasmid

    #247035

    Purpose

    IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    IRF1

    Available Since

    Nov. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Px-PhiYFP-triplex-28-M13-28-pA

    Plasmid

    #202047

    Purpose

    Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    PhiYFP
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Px-PhiYFP-triplex-DR-M13-DR-pA

    Plasmid

    #202046

    Purpose

    Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    PhiYFP
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-2G-CNCB

    Plasmid

    #236764

    Purpose

    A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter, human U6 promoter, CAG promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQGG005

    Plasmid

    #231332

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI-ELT4 counterselectable site for BpiI cloning. L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge