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pAAV-FLEX-SaCas9-U6-sgGria3

Plasmid

#124855

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

Plasmid

#187154

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG-FGFRdn

Plasmid

#80431

Purpose

Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Fibroblast growth factor receptor 1 (FGFR1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1–425

Promoter

beta-actin promoter

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only
HR700_HA2TP53_Ex5

Plasmid

#228860

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex6

Plasmid

#228861

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex7

Plasmid

#228862

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex8

Plasmid

#228863

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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BRD4 gRNA (BRDN0001147320)

Plasmid

#77344

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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