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sTR060
Plasmid#177370PurposeTrigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.DepositorInsertTrigger RNA
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK021-7-PARS
Plasmid#84990PurposeType 7, Pichia autonomously replicating sequenceDepositorInsertPichia autonomously replicating sequence
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK017-3b-yEGFP_Sec
Plasmid#84976PurposeType 3b, Green fluorescent proteinDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK015-3-yEGFP_Intra
Plasmid#84974PurposeType 3, Green fluorescent proteinDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK019-4-tAOX1
Plasmid#84988PurposeType 4, tAOX, Alcohol oxidase 1 terminatorDepositorInsertAlcohol oxidase 1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
sTR056
Plasmid#177369PurposeToehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK021-7-PARS
Plasmid#84990PurposeType 7, Pichia autonomously replicating sequenceDepositorInsertPichia autonomously replicating sequence
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK017-3b-yEGFP_Sec
Plasmid#84976PurposeType 3b, Green fluorescent proteinDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK015-3-yEGFP_Intra
Plasmid#84974PurposeType 3, Green fluorescent proteinDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK019-4-tAOX1
Plasmid#84988PurposeType 4, tAOX, Alcohol oxidase 1 terminatorDepositorInsertAlcohol oxidase 1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pPTK009-3a-αAmylase-αMFΔ
Plasmid#84968PurposeType 3a, α-Amylase followed by αMFΔ - secretion signalDepositorInsertα-Amylase followed by MFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK010-3a-Glucoamylase-αMFΔ
Plasmid#84969PurposeType 3a, Glucoamylase followed by αMFΔ - secretion signalDepositorInsertGlucoamylase followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK006-3a-αMF no EAEA
Plasmid#84965PurposeType 3a, α-mating factor no EAEA - secretion signalDepositorInsertα-mating factor no EAEA secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK001-2-pAOX1
Plasmid#84960PurposeType 2, pAOX1, Alcohol oxidase 1 promoterDepositorInsertAlcohol oxidase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only
