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Showing: 741 - 760 of 1511 results

  1. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172826-plasmid-map-sequence-id-340920

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  2. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  5. pExT

    Plasmid
    #36889
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides before nucleotide 275 and ins…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    36889-plasmid-map-sequence-id-46194

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  6. pExG

    Plasmid
    #36888
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36888-plasmid-map-sequence-id-46193

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Showing: 741 - 760 of 1511 results