We narrowed to 1,931 results for: cas9 expression vector

1. pCfB3044(gRNA XI-2)

   Plasmid

   #73286

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB3048(gRNA XII-2)

   Plasmid

   #73290

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3043(gRNA XI-1)

   Plasmid

   #73285

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB3049(gRNA XII-4)

   Plasmid

   #73291

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00023 (aka pRC0434)

   Plasmid

   #195309

   Purpose

   Lentiviral expression vector derived from LEGO-G2 with G418/Neomycin selection

   Depositor

   Raj Chari

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPi Kit

   Plasmid Kit

   #1000000136

   Purpose

   CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

   Depositor

   Brigitte Gasser

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pLV.5’eGFP/sgVIM

   Plasmid

   #170548

   Purpose

   Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   sgRNA targeting 5'- end of VIM
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAJAK-71 (pESC-NatMXsyn-USER)

   Plasmid

   #78232

   Purpose

   Empty vector, for the insertion of gRNA expression cassettes into the vector.

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV.5’VIM-eGFP-KI

    Plasmid

    #170547

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for VIM knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWS158

    Plasmid

    #90517

    Purpose

    Cas9 gap repair vector - URA3

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWS175

    Plasmid

    #90962

    Purpose

    Cas9 gap repair vector - HygR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWS172

    Plasmid

    #90519

    Purpose

    Cas9 gap repair vector - HIS3

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS171

    Plasmid

    #90518

    Purpose

    Cas9 gap repair vector - LEU2

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWS174

    Plasmid

    #90961

    Purpose

    Cas9 gap repair vector - NatR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS176

    Plasmid

    #90963

    Purpose

    Cas9 gap repair vector - ZeoR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

    Plasmid

    #179003

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge