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GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Article

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pGEX/PD-L1 WT

Plasmid

#121466

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ICD

Plasmid

#121480

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Mutation

TM+ICD

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ECD

Plasmid

#121479

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Mutation

ECD+TM

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PD-L1 3SA

Plasmid

#121493

Purpose

Expression of human PD-L1 3SA in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 (CD274 Human)

Tags

HA

Expression

Mammalian

Mutation

S176A, T180A, S184A

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SARS-CoV-2_PLP

Plasmid

#203509

Purpose

Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 3SA

Plasmid

#121467

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Mutation

S176A, T180A, S184A

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-FES

Plasmid

#203501

Purpose

Expresses human FES kinase from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

FES (FES Human)

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-c-Src

Plasmid

#203462

Purpose

Expresses human c-Src kinase from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

c-Src (CSK Human)

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-SARS-CoV-2_3CL

Plasmid

#203496

Purpose

Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-Bat-CoV-HKU9_3CL

Plasmid

#203478

Purpose

Expresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-CoV HKU9 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-2x-uA-BWCoV-SW1_3CL

Plasmid

#201936

Purpose

Expresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Beluga Whale-CoV SW1 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-CoV-HKU4_3CL

Plasmid

#201943

Purpose

Expresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-CoV HKU4 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-229E_3CL

Plasmid

#201937

Purpose

Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-229E 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-NL63_3CL

Plasmid

#201938

Purpose

Expresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTCFL5.1.0-gDNA

Plasmid

#113775

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TCFL5

Depositor

Kevin White

Insert

TCFL5 (TCFL5 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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XanthoMoClo Xanthobacter Genetic Toolkit

Plasmid Kit

#1000000259

Purpose

Use this modular cloning kit to assemble plasmids for expression in Xanthobacter and Roseixanthobacter.

Depositor

Michael Springer

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only
pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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