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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,143 results for: fret

Showing: 741 - 760 of 1143 results

  1. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  2. pcDNA-mito-ZifCV1.49

    Plasmid
    #59009
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZifCV1.49 genetically encoded Zn(II) sensor
    Tags
    See comment
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  5. pcDNA-mito-ZifCV1.173

    Plasmid
    #59010
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZifCV1.173 genetically encoded Zn(II) sensor
    Tags
    See comment
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pUC-GW-Kan-VPC

    Plasmid
    #244163
    Purpose
    Reporter for human p38alpha activation state by FRET
    Depositor
    Thomas Kuhlman
    Insert
    MAPK14/p38a (MAPK14 Human)
    Use
    Synthetic Biology
    Tags
    mCerulean3 and mVenus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    244163-plasmid-map-sequence-id-487534

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  8. Epac-S-H127

    Plasmid
    #170343
    Purpose
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170343-plasmid-map-sequence-id-344642

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  9. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125200-plasmid-map-sequence-id-243591

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Showing: 741 - 760 of 1143 results