Addgene

We narrowed to 1,261 results for: membrane gfp

Showing: 741 - 760 of 1261 results

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YIplac211-SNX4-iGFPx3

Plasmid

#105269

Purpose

Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snx4 portion (SNX4 Budding Yeast)

Use

Tags

iGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-RCY1

Plasmid

#105281

Purpose

Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Rcy1 (RCY1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-HSE1-iGFPx6

Plasmid

#105259

Purpose

Plasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Hse1 portion (HSE1 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHS3-msGFPx3

Plasmid

#105257

Purpose

Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chs3 portion (CHS3 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS16-iGFPx6

Plasmid

#105275

Purpose

Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps16 portion (VPS16 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pETSyt1C2AB(80-421)msGFP

Plasmid

#209009

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80-421)msGFP

Plasmid

#209014

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-UTR

Plasmid

#121142

Purpose

Mammalian expression of GFP tagged CD274 with its own 3'UTR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

CD274 with its own 3'UTR (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Daniel Hammer, Benjamin Schuster, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Daniel Hammer, Benjamin Schuster, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

EGFP and His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
PGK-GFP-LAMP1TM

Plasmid

#234874

Purpose

Lentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM.

Depositor

Andrew Arrant

Article

Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

Insert

EGFP

Use

Lentiviral

Tags

LAMP-1 transmembrane domain and cytosolic tail

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Daniel Hammer, Benjamin Schuster, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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CIBN-GFP-TMD

Plasmid

#79571

Purpose

expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

Depositor

Olof Idevall-Hagren, Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

syntaxin 1A (Stx1a Mouse)

Use

Tags

CIBN and EGFP

Expression

Mammalian

Mutation

aa 249_288 (C-terminal region (polybasic stretch …

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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2C-EGFP-t2a-mCD4

Plasmid

#216213

Purpose

Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

Depositor

Michelle Percharde

Article

Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

Insert

CD4 (Cd4 Mouse)

Use

Tags

GFP-T2A

Expression

Mammalian

Mutation

CD4 transmembrane domain only

Promoter

MERVL

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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