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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,670 results for: nif

Showing: 741 - 760 of 1670 results

  1. pOpen-NcoIM

    Plasmid
    #165578
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.NcoI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 3, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165578-plasmid-map-sequence-id-338826

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  3. pOpen-XbaIM

    Plasmid
    #165577
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.XbaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165577-plasmid-map-sequence-id-341004

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  4. pOpen-Bst HF

    Plasmid
    #165539
    Purpose
    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Bst HF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165539-plasmid-map-sequence-id-339129

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  5. pOpen-9N7lig

    Plasmid
    #165514
    Purpose
    9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C).
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    9 Degrees North DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165514-plasmid-map-sequence-id-338640

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  6. pOpen-T4-BGT

    Plasmid
    #165530
    Purpose
    T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T4-BGT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165530-plasmid-map-sequence-id-339085

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  7. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Eco31I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

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  8. pOpen-9N7polA

    Plasmid
    #165500
    Purpose
    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    9 degrees N-7 DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165500-plasmid-map-sequence-id-338428

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  9. pOpen-THEAQlig

    Plasmid
    #165535
    Purpose
    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Taq DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165535-plasmid-map-sequence-id-339125

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  10. pOpen-UDG

    Plasmid
    #165531
    Purpose
    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    UDG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165531-plasmid-map-sequence-id-339117

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  11. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

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  12. pOpen-TthpolA

    Plasmid
    #165533
    Purpose
    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Tth DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165533-plasmid-map-sequence-id-339122

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  13. pWPI-myogenin

    Plasmid
    #79073
    Purpose
    Lentiviral plasmid that constitutively expresses human myogenin
    Depositor
    Jennifer Lei
    Insert
    human myogenin (MYOG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available Since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79073-plasmid-map-sequence-id-151242

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  14. pOpen-T4 PGD

    Plasmid
    #165550
    Purpose
    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T4 PGD (T4 Endonuclease V)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165550-plasmid-map-sequence-id-339867

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  15. pOpen-DpnIR

    Plasmid
    #165529
    Purpose
    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DpnI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165529-plasmid-map-sequence-id-339077

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  16. pOpen-SP6rpol

    Plasmid
    #165516
    Purpose
    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    SP6 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165516-plasmid-map-sequence-id-338647

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  17. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    TEV protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

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  18. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  19. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  20. pOpen-ALPI

    Plasmid
    #165555
    Purpose
    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    CIP (calf intestinal phosphatase)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165555-plasmid-map-sequence-id-339907

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Showing: 741 - 760 of 1670 results