We narrowed to 5,301 results for: pcDNA3.1+

1. pcDNA3.1-GGamma8-GFP2

   Plasmid

   #140990

   Purpose

   Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma8-GFP2 (GNG8 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) K-Ras(G12C)

   Plasmid

   #224284

   Purpose

   Cell transfection and expression of K-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   K-Ras (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1+_FH-AGO2-WT

   Plasmid

   #92006

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

   Plasmid

   #114595

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-3xHA-mASL

   Plasmid

   #34577

   Purpose

   NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.

   Depositor

   Peter Murray

   Insert

   ASL (Asl Mouse)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Hyg-RFPKDEL

   Plasmid

   #138660

   Purpose

   Reporter plasmid expressing the ER-located RFP

   Depositor

   Bertrand Collet

   Article

   Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

   Insert

   RFP-KDEL
   

   Use

   Tags

   KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HsRRP6_Z

   Plasmid

   #148131

   Purpose

   Mammalian Expression of HsRRP6

   Depositor

   Elisa Izaurralde

   Insert

   HsRRP6 (EXOSC10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 silent mutations compared to the sequence of is…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-CHRNA7-mCherry

   Plasmid

   #62632

   Purpose

   mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   hAlpha7 (CHRNA7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry inserted into M3-M4 loop

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-TEK-V5/HIS

   Plasmid

   #205611

   Purpose

   Expression of human TEK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TEK receptor tyrosine kinase (TEK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hSTING (R232)

    Plasmid

    #214152

    Purpose

    Transient expression of STING R232

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PDI-QUAD-V5-pcDNA3.1

    Plasmid

    #153550

    Purpose

    PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

    Depositor

    Julie Atkin

    Article

    Parakh et al iScience. 2020 May 22;23(5):101097. doi:

    Insert

    Protein disulphide isomerase
    

    Use

    Tags

    V5 tag

    Expression

    Mutation

    C53S, C56S, C397S, C400S

    Promoter

    pCMV

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-GGamma13-GFP2

    Plasmid

    #140992

    Purpose

    Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma13-GFP2 (GNG13 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 myc TSC1

    Plasmid

    #12133

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. opto-RhoGEF-mCherry_pcDNA3.1

    Plasmid

    #164473

    Purpose

    Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoGEF
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3.1-nv5-ER beta

    Plasmid

    #22770

    Depositor

    Donald McDonnell

    Article

    Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

    Insert

    estrogen receptor beta (ESR2 Human)
    

    Use

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1/Zeo-CnB

    Plasmid

    #191965

    Purpose

    Expression of CnB (calcineurin subunit beta).

    Depositor

    Luca Scorrano

    Article

    Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

    Insert

    Calcineurin subunit beta (PPP3CB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-HsPcdh21-mNeonGreen

    Plasmid

    #210819

    Purpose

    Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Protocadherin 21 (CDHR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-MS2-HA-HsRck_AC

    Plasmid

    #148417

    Purpose

    Mammalian Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-mTagBFP2-MRTF-A

    Plasmid

    #182061

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-A (MRTFA Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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